The Laser Molecular Imaging and Machine Learning laboratory team jointly with colleagues from Manipal Academy of Higher Education, Manipal, India, University of Zurich, Switzerland, Indian Institute of Technology, Ropar, India, National Yang Ming Chiao Tung University, Taipei, Taiwan published an article titled “Two-photon fluorescence and second-harmonic generation microscopy: principles and biomedical applications” in the journal “Journal of biophotonics”. (Q2).
Two-photon microscopy (TPM) has revolutionized biological imaging by enabling high-resolution, deep-tissue visualization with reduced photobleaching and photodamage. This review discusses complementary nonlinear optical imaging modalities such as two-photon fluorescence (TPF), second-harmonic generation (SHG), and fluorescence lifetime imaging (FLIM) microscopy that Подробнее




